Acute peritonitis: adhesion molecules are regulated by a balance between endogenous and exogenous Annexin A1

Autores

  • Cristiane Damas Gil
  • Caio Eduardo Gullo
  • Sonia Maria Oliani

Resumo

This study evaluated the effect of endogenous and exogenous annexin A1 (ANXA1) on cell
migration through the modulation of β2-integrin (CD11b) and L-selectin (CD62L) adhesion
molecules, using a classic model of peritonitis. Wild type (WT) and ANXA1 null (ANXA1null)
mice, pretreated or not with the peptide Ac2-26 (ANXA1 N-terminal; 100 μg) received i.p.
administration of zymosan (1.0 mg). After, blood, peritoneal exudates and mesenteries were
evaluated by biochemical and cellular analysis at 0, 4 and 24 h. Exogenous administration of Ac2-
26 significantly decreased polymorphonuclear cells (PMN) trafficking and TNF-a release 4 h after
the peritonitis process. In the inflammation resolution phase (24h), no difference was observed
between animals treated with Ac2-26 or not. In addition to the change in cell recruitment,
pretreatment with Ac2-26 significantly increased the expression of L-selectin and β2-integrin in
blood PMN, but no effect was observed in these cells on ANXA1null. Ultrastructural analysis
showed co-localization between ANXA1 and adhesion molecules, particularly CD11b, in the
plasma membrane and cytoplasm of neutrophils and endothelial cells. Administration of Ac2-26
significantly reduced CD11b levels in these cells, but ANXA1null neutrophils had a high
proportion of CD26L that was not modulated by the exogenous peptide. We envisage that the
endogenous levels of ANXA1 are important to control the inflammatory response through CD62L
in PMN, whereas exogenous ANXA1 regulates the release of pro-inflammatory cytokines and
CD11b in PMN and endothelial cells.

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Publicado

2021-08-30

Como Citar

Damas Gil, C., Eduardo Gullo, C., & Maria Oliani, S. (2021). Acute peritonitis: adhesion molecules are regulated by a balance between endogenous and exogenous Annexin A1. ULAKES JOURNAL OF MEDICINE, 1(2). Recuperado de https://revistas.unilago.edu.br/index.php/ulakes/article/view/481

Edição

v. 1 n. 2 (2021): Produção científica em meio à pandemia

Seção

Artigos

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Direitos Autorais:  Os direitos autorais para os artigos publicados são reservados à ULAKES Journal of Medicine. Em virtude de tratar-se de um periódico de acesso aberto, que utiliza a licença Creative Commons Attribution (CC BY-NC-ND 4.0), é permitido o uso gratuito dos artigos, principalmente em aplicações educacionais e científicas, desde que citada a fonte original. 

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